Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E R169Q | mutant protein is strongly overexpressed with accumulation of proteolytic fragments | Escherichia coli |
additional information | viable mutations affecting the 5'-phosphate sensor of RNase E, including R169Q or T170A, become lethal when combined with deletions removing part of the non-catalytic C-terminal domain of RNase E. Mutation of the phosphate sensor stabilizes the rpsT P1 mRNA as much as sixfold and slows the maturation of 16S rRNA. In contrast, the decay of other model mRNAs and the processing of several tRNA precursors are unaffected by mutations in the phosphate sensor | Escherichia coli |
additional information | deletion of residues C-terminal to position 529 in the absence of other mutations still permit growth of cells. Deletion strain exhibits smaller colony size and reduced growth rates in liquid media. Additional shorter deletions spanning individual microdomains in the C-terminal scaffold region including the Arg-rich region, residues 608-644, the extended Arg-rich region with a putative coil-coil domain, residues 589-723, the RhlB binding site, residues 698762, the enolase binding site, residues 833-850, or the PNPase binding site, residues 1021-1061, are viable, too | Escherichia coli |
R1269Q/DELTA530-1061 | mutation is lethal | Escherichia coli |
R1269Q/DELTA589-1061 | mutation is lethal | Escherichia coli |
R1269Q/DELTA730-1061 | mutant is viable | Escherichia coli |
R169Q | site-directed mutagensis, the viable mutation in the 5'-phosphate sensor of RNase E, becomes lethal in combination with deletions removing part of the non-catalytic C-terminal domain of RNase E. Loss of autoregulation in R169Q | Escherichia coli |
T170A | site-directed mutagensis, the viable mutation in the 5'-phosphate sensor of RNase E, becomes lethal in combination with deletions removing part of the non-catalytic C-terminal domain of RNase E | Escherichia coli |
T170A/DELTA530-1061 | mutant is viable, with small colony sizes | Escherichia coli |
T170A/DELTA589-1061 | mutant is viable, with small colony sizes | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | P21513 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
RNase E | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the phosphate sensor domain in the enzyme is required for efficient autoregulation of RNase E synthesis. Impact of the 5'-sensor on mRNA stability, overview | Escherichia coli |
physiological function | viable mutations affecting the 5'-phosphate sensor of RNase E, including R169Q or T170A, become lethal when combined with deletions removing part of the non-catalytic C-terminal domain of RNase E. The phosphate sensor is required for efficient autoregulation of RNase E synthesis as RNase E R169Q is strongly overexpressed with accumulation of proteolytic fragments. In addition, mutation of the phosphate sensorstabilizes the rpsT P1 mRNA as much as sixfold and slows the maturation of 16S rRNA. The decay of other model mRNAs and the processing of several tRNA precursors are unaffected by mutations in the phosphate sensor | Escherichia coli |